02024nas a2200385   4500000000100000008004100001260001300042653001200055653002400067653003800091653001300129653001600142653001100158653001700169653002100186653002100207653001200228653001100240653002900251653002400280653002500304653003200329653001200361653002000373100001200393700001900405700001500424700001500439700001600454245011600470300001200586490000700598520101900605022001401624       1983                            d  c1983 Sep10aAnimals10aAntigens, Bacterial10aEnzyme-Linked Immunosorbent Assay10aEpitopes10aGlycolipids10aHumans10aImmunization10aImmunoglobulin G10aImmunoglobulin M10aleprosy10aLipids10aMycobacterium Infections10aMycobacterium bovis10aMycobacterium leprae10aNontuberculous Mycobacteria10aRabbits10aSerologic Tests1 aCho S N1 aYanagihara D L1 aHunter S W1 aGelber R H1 aBrennan P J00aSerological specificity of phenolic glycolipid I from Mycobacterium leprae and use in serodiagnosis of leprosy.  a1077-830 v413 a<p>The serological activities of the specific phenolic glycolipid I from Mycobacterium leprae, its dissected parts, and related glycolipids from other mycobacteria were examined by enzyme-linked immunosorbent assay against hyperimmune anti-M. leprae rabbit antiserum and sera from patients with leprosy and other mycobacterial diseases. High anti-phenolic glycolipid I immunoglobulin M antibodies were found in 23 of 24 (96%) of lepromatous leprosy patients on short term chemotherapy and in 8 of 13 tuberculoid leprosy patients (62%). Sera from patients with tuberculosis or atypical mycobacterial infections were devoid of anti-phenolic glycolipid I activity. The structurally related phenolic glycolipids from Mycobacterium kansasii and Mycobacterium bovis and the aglycone segments of the M. leprae product showed no significant activity. Thus, the trisaccharide determinant of phenolic glycolipid I is specific in its structure, serological activity, and, to a lesser extent, the antibody class it evokes.</p>  a0019-9567