02366nas a2200445   4500000000100000008004100001260001300042653001200055653002400067653002300091653001400114653002000128653002000148653001100168653001200179653002600191653000900217653002100226653002100247653002400268653002500292653002200317653002400339100001800363700001800381700001500399700001200414700001200426700001500438700001600453700001300469700001300482700001300495245010900508856007200617300001100689490000700700520119900707022001401906       1999                            d  c1999 Mar10aAnimals10aAntigens, Bacterial10aBacterial Proteins10aCytokines10aHLA-DR Antigens10aHLA-DRB1 Chains10aHumans10aleprosy10aLymphocyte Activation10aMice10aMice, Transgenic10aMolecular Weight10aMycobacterium avium10aMycobacterium leprae10aPeptide Fragments10aSpecies Specificity1 aWilkinson R J1 aWilkinson K A1 aJurcevic S1 aHills A1 aSinha S1 aSengupta U1 aLockwood DN1 aKatoch K1 aAltman D1 aIvanyi J00aSpecificity and function of immunogenic peptides from the 35-kilodalton protein of Mycobacterium leprae.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC96486/pdf/ii001501.pdf  a1501-40 v673 a<p>We identified a T-cell determinant of the 35-kDa antigen of Mycobacterium leprae which is discriminatory against cross-sensitization by its closely related homologue in Mycobacterium avium. From synthetic peptides covering the entire sequence, those with the highest affinity and permissive binding to purified HLA-DR molecules were evaluated for the stimulation of proliferation of peripheral blood mononuclear cells (PBMCs) from leprosy patients and healthy sensitized controls. Responses to the peptide pair 206-224, differing by four residues between M. leprae and M. avium, involved both species-specific and cross-reactive T cells. Lymph node cell proliferation in HLA-DRB1*01 transgenic mice was reciprocally species specific, but only the response to the M. leprae peptide in the context of DR1 was immunodominant. Of the cytokines in human PBMC cultures, gamma interferon production was negligible, while interleukin 10 (IL-10) responses in both patients and controls were more pronounced. IL-10 was most frequently induced by the shared 241-255 peptide, indicating that environmental cross-sensitization may skew the response toward a potentially pathogenic cytokine phenotype.</p>  a0019-9567