01301nas a2200301   4500000000100000008004100001260001300042653001500055653001000070653002400080653001100104653001100115653003000126653001300156653001200169653000900181653001600190653002400206100001500230700001100245700001600256245007500272300001100347490000700358050003200365520058800397022001400985       1979                            d  c1979 Jul10aAdolescent10aAdult10aAntigens, Bacterial10aFemale10aHumans10aHypersensitivity, Delayed10aLepromin10aleprosy10aMale10aMiddle Aged10aReference Standards1 aSengupta U1 aRamu G1 aDesikan K V00aAssessment of Dharmendra antigen. II. Standardisation of the antigens.  a316-220 v51  aInfolep Library - available3 a<p>Dharmendra antigen with different bacterial counts (16, 12.5, 10, 7.5, 5 and 2.5 million/ml) have been utilized for determination of skin delayed hypersensitivity in leprosy patients. It has been noted that antigen with 10 million acid fast bacilli (AFB)/ml mounts a standard early (24 hrs) as well as late (3 weeks) reaction in patients. Lepromatous patients do not show any skin reaction with this dilution. Thus, a standard Dharmendra antigen has been prepared using a considerably smaller number of organisms as compared to the International standards for Mitsuda antigen.</p>  a0024-1024