01531nas a2200325   4500000000100000008004100001260001300042653001200055653002300067653001900090653003100109653001600140653001700156653001600173653003800189653001100227653001200238653002400250653001200274653002000286653001000306653001700316100001600333700001800349245012800367300001100495490000800506520067700514022001401191       1974                            d  c1974 Dec10aAnimals10aAntibody Formation10aChromatography10aChromatography, Thin Layer10aCoombs Test10aErythrocytes10aGlycolipids10aHemagglutination Inhibition Tests10aHumans10aleprosy10aMycobacterium bovis10aRabbits10aSerologic Tests10aSheep10aTuberculosis1 aReggiardo Z1 aMiddlebrook G00aSerologically active glycolipid families from Mycobacterium bovis BCG. I. Extraction, purification and immunologic studies.  a469-760 v1003 a<p>Glycolipids were extracted from mycobacteria with methanol and chloroform and purified by silicic acid chromatography. These glycolipids were studied for their serologic activity by direct and indirect (Coomb's) passive hemagglutination, and by inhibition methods. Three families of serologically active glycolipids called A, B and C, plus cardiolipin, were isolated. The B and C families of glycolipids were reactive with sera from BCG immunized rabbits and also with sera from patients with tuberculosis and leprosy; the A family was reactive only with the human sera. None of the serologically active glycolipids was able to protect rabbits against tuberculosis.</p>  a0002-9262