01214nas a2200241   4500000000100000008004100001260000900042653000900051653003100060653002100091653001100112653001200123653002500135653001600160100001600176700001400192245009300206856008300299300001200382490000700394520055700401022001400958       1972                            d  c197210aAgar10aBacteriological Techniques10aCell-Free System10aHumans10aleprosy10aMycobacterium leprae10aTemperature1 aMurohashi T1 aYoshida K00aAn attempt to culture Mycobacterium leprae in cell-free, semisynthetic, soft agar media.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2480898/pdf/bullwho00182-0055.pdf  a195-2100 v473 a<p>This paper describes an attempt to culture M. leprae from leprous nodules, using cell-free, semisynthetic, soft agar media, and the effects of varying the incubation temperature and the composition of the basic medium, as well as of pretreating the bacterial suspensions with alkali. The identification test carried out in leprosy patients by intradermal injection of bacillary suspensions prepared from the primary cultures and third subcultures suggested that the bacterial masses that had formed in the suspensions were composed of M. leprae.</p>  a0042-9686