01993nas a2200241   4500000000100000008004100001260000900042653001200051653002600063653002400089653002200113653001100135653002400146653002800170100001400198700001200212700001300224245010500237300001000342490000700352520137800359022001401737       1999                            d  c199910aAnimals10aAntibodies, Protozoan10aAntigens, Protozoan10aBlotting, Western10aHumans10aLeishmania donovani10aLeishmaniasis, Visceral1 aSalotra P1 aRaina A1 aNegi N S00aImmunoblot analysis of the antibody response to antigens of Leishmania donovani in Indian kala-azar.  a263-70 v563 a<p>When infected with Leishmania donovani, patients develop specific antibodies that constitute the basis of serodiagnosis. Using immunoblot analysis, we examined the antibody response to antigens of L. donovani in 35 kala-azar (KA) patients and 67 controls. Sera from KA patients recognised numerous antigens with molecular weights ranging from 14-110 kDa. Antigens of 40 kDa, 55 kDa, 65 kDa, 70 kDa and 82 kDa were recognised most frequently. All KA patients produce an antibody response to one or more of these antigens. The majority (83%) of KA cases recognised at least four of these five parasite antigens. The 70 kDa antigen showed the greatest sensitivity for Indian KA, and produced a positive reaction in 94% of patients. This antigen gave 10% false-positive reactions in controls comprising patients with related diseases (i.e. tuberculosis, leprosy and malaria) and in healthy controls. Data indicated that the 70 kDa antigen may include a member of the heat shock protein 70 family. Studies with four clinical isolates of L. donovani showed that the 70 kDa component was expressed in all the strains examined. Immunoblot assay (Western blotting) provided a sensitive diagnostic test for KA patients, and identified the 70 kDa parasite antigen that is promising as a potential target antigen for the development of less complex serodiagnostic assays for KA.</p>  a0967-4845