02031nas a2200373   4500000000100000008004100001260000900042653001200051653003100063653001800094653001400112653002000126653001800146653002300164653001200187653001500199653001100214653001200225653001200237653001600249653000900265653002500274653001800299653002500317653003100342653000900373100001500382245012000397856008300517300001100600490000700611520102500618022001401643       1973                            d  c197310aAnimals10aBacteriological Techniques10aCell Division10aCell Line10aCells, Cultured10aCulture Media10aCulture Techniques10aGanglia10aHaplorhini10aHumans10aL Forms10aleprosy10aMacrophages10aMice10aMicroscopy, Electron10aMycobacterium10aMycobacterium leprae10aMycobacterium lepraemurium10aRats1 aPattyn S R00aThe problem of cultivation of Mycobacterium leprae. A review with criteria for evaluating recent experimental work.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2480944/pdf/bullwho00171-0074.pdf  a403-100 v493 a<p>Some criteria are presented to help evaluate papers appearing in the literature claiming successful cultivation of M. leprae either in the absence or in the presence of tissue-cultured cells. Recently, electron microscopic studies have definitely shown M. leprae to belong to the genus Mycobacterium and its division to occur through transverse section. A survey is given of the mycobacterial strains isolated in the last 10 years from leprosy lesions. These strains belong to taxonomically different species and cannot be considered to be M. leprae. No substantiated claim was made concerning the in vitro growth of M. leprae and the application of the tissue culture technique has been equally disappointing. The view is expressed that progress towards the in vitro cultivation of M. leprae can be made only as a result of increased knowledge about the intracellular environment and the metabolic activities of this organism, to be obtained by the application of modern biochemical and histochemical techniques.</p>  a0042-9686