01642nas a2200313   4500000000100000008004100001260001300042653001200055653002600067653002400093653001600117653001100133653001600144653002600160653001200186653001200198653002500210653003100235653001200266100001600278700001300294700001200307700001200319245006600331300001000397490000600407520090100413022001401314       1974                            d  c1974 May10aAnimals10aAntibodies, Bacterial10aAntigens, Bacterial10aBCG Vaccine10aHumans10aImmune Sera10aImmunoelectrophoresis10aleprosy10aMethods10aMycobacterium leprae10aMycobacterium tuberculosis10aRabbits1 aAxelsen N H1 aHarboe M1 aCloss O1 aGodal T00aBCG antibody profiles in tuberculoid and lepromatous leprosy.  a952-80 v93 a<p>In sera from 12 patients with polar tuberculoid leprosy, 12 with subpolar tuberculoid leprosy, and 16 with lepromatous leprosy were demonstrated a total number of 125 anti-BCG precipitins by means of crossed immunoelectrophoresis with intermediate gel. Up to 14 different precipitins were found in individual sera, and the complexity in antibody response was higher than previously realized. The specificity of 69% of the antibodies was defined, and these antibodies were titrated in three arbitrary titer units. A highly significant difference (P < 0.002) was found in antibody response between the tuberculoid and the lepromatous group. Due to simplicity, sensitivity, and high resolution, the method used is a promising tool for providing exact data to be used as guidelines for purification of important individual mycobacterial antigens. The need for reference antisera is emphasized.</p>  a0019-9567