02125nas a2200277   4500000000100000008004100001260001300042653002400055653001400079653002000093653002000113653001400133653004300147653001800190653003100208653002100239100001600260700001300276700001500289700001300304245010800317300001000425490000800435520139000443022001401833       1985                            d  c1985 Jan10aAntigens, Bacterial10aCell Wall10aCorynebacterium10aCross Reactions10aCytoplasm10aImmunoelectrophoresis, Two-Dimensional10aMycobacterium10aPolysaccharides, Bacterial10aRadioimmunoassay1 aAbou-Zeid C1 aHarboe M1 aSundsten B1 aCocito C00aCross-reactivity of antigens from the cytoplasm and cell walls of some corynebacteria and mycobacteria.  a170-80 v1513 a<p>Leprosy-derived corynebacteria (LDC) are non-acid-fast organisms isolated from leprosy lesions in humans. In this study 20 antigens of native LDC cytoplasm were identified by immunoelectrophoresis, and autoclaving yielded the M1 component, which strongly cross-reacted with antigen 60 of Mycobacterium bovis BCG (bacille Calmette-Guérin) and antigen 7 of Mycobacterium leprae. The polysaccharide moiety of M1 was immunologically related to the LDC cell wall polysaccharide previously characterized as arabinogalactomannan. The latter polysaccharide competitively inhibited the formation of immune complexes by labeled M1 and antisera to the LDC cell wall; cytoplasm and wall polysaccharides from other bacteria produced lower-level inhibition. In a radioimmunoassay with 125I-labeled antigen 7 of M. leprae, sera from patients with leprosy and antisera to the LDC cell wall yielded overlapping curves. Sera from patients with tuberculoid leprosy and those from patients with lepromatous leprosy afforded different levels of inhibition in this radioimmunoassay; this result indicated a difference in antibody specificity in the two forms of leprosy. In conclusion, the cell wall polysaccharide of LDC corresponds to the main thermostable cytoplasmic antigen M1, which strongly crossreacts with sera from patients with leprosy and, more specifically, with antigen 7 of M. leprae.</p>  a0022-1899