02142nas a2200325   4500000000100000008004100001260001700042653002700059653002400086653001400110653004100124653001100165653002800176653002100204653001300225653001200238653001600250653000900266100001800275700001100293700001700304700001500321700001600336245011400352300001100466490000700477050003200484520128600516022001401802       1985                            d  c1985 Apr-Jun10aAntibodies, Monoclonal10aAntigens, Bacterial10aGranuloma10aHistocompatibility Antigens Class II10aHumans10aImmunoenzyme Techniques10aLangerhans Cells10aLepromin10aleprosy10aLymphocytes10aSkin1 aNarayanan R B1 aRamu G1 aMalaviya G N1 aSengupta U1 aDesikan K V00aIn situ characterization of cells in the dermal infiltrates of lepromin reaction using monoclonal antibodies.  a265-720 v57  aInfolep Library - available3 a<p>A study was made on the in situ characteristics of dermal infiltrates in the early and late lepromin reaction with monoclonal antibodies defining T cell subsets, Langerhan cells and Ia like antigens. The early reaction (24 hrs) was elicited either with standard Dharmendra lepromin or leprosin-A and the late reaction (3-4 weeks) was elicited with standard Dharmendra lepromin. In all, 15 biopsies were studied. Most lymphocytes in the infiltrates of both the lepromin and leprosin reactions were positive for OKT 11, Leu 3a, OKT 8 and Ia like antigens indicating thereby the presence of activated T cells. A high proportion of OKT6 + cells were also noticed in the infiltrates of these reactions. In the late reaction, the lymphocytes in the granulomas were predominantly activated T lymphocytes expressing OKT 11, Leu 3a, OKT 8 and Ia like antigens. Leu 3a + cells were scattered diffusely amidst the epithelioid cells. In contrast, OKT 8 + cells were present mainly in the peripheral region of the granuloma. A small proportion of OKT6 + cells were also seen in these granulomas. Ia like antigens and T6 antigens were not discernible on the epithelioid cells. No difference in the number of OKT6 + epidermal langerhan cells was observed in the various types of reactions.</p>  a0254-9395