01221nas a2200265   4500000000100000008004100001260001600042653002400058653001600082653001100098653002900109653001200138653002600150653002500176653001300201653001300214100001400227700001300241700001700254245011300271300001100384490000700395520053900402022001400941       1985                            d  c1985 May 2310aAntigens, Bacterial10aGlycolipids10aHumans10aImmunosorbent Techniques10aleprosy10aMembranes, Artificial10aMycobacterium leprae10aPolymers10aSulfones1 aYoung D B1 aFohn M J1 aBuchanan T M00aUse of a polysulfone membrane support for immunochemical analysis of a glycolipid from Mycobacterium leprae.  a205-110 v793 a<p>Polysulfone membranes have been used as a solid support for chromatography and immunoblotting of phenolic glycolipid I from Mycobacterium leprae. These membranes have an advantage over other supports such as nitrocellulose and silica gel in that very little non-specific background binding of antibodies occurs and assays can readily be carried out with IgM antibodies from human sera. An example of use of the polysulfone chromatography system for detection of phenolic glycolipid I in sera from leprosy patients is described.</p>  a0022-1759