01806nas a2200349   4500000000100000008004100001260001300042653001000055653002700065653001000092653001100102653002000113653001400133653002000147653004100167653001100208653001200219653000900231653001200240653001600252653002000268653000900288653002200297100001700319700001300336700001600349245011600365300001000481490000700491520094400498022001401442       1985                            d  c1985 Jul10aAdult10aAntibodies, Monoclonal10aChild10aFemale10aGlucose Oxidase10aGranuloma10aHLA-DR Antigens10aHistocompatibility Antigens Class II10aHumans10aleprosy10aMale10aMethods10aMiddle Aged10aPalatine Tonsil10aSkin10aSpectrophotometry1 aCollings L A1 aTidman N1 aPoulter L W00aQuantitation of HLA-DR expression by cells involved in the skin lesions of tuberculoid and lepromatous leprosy.  a58-660 v613 a<p>A method is described which can be used to quantitate class II MHC antigens (HLA-DR) expressed by cells within tissue sections. A mouse anti-human HLA-DR monoclonal antibody is directly conjugated to the fungal enzyme glucose oxidase. The enzyme, in the presence of its substrate can be used to reduce tetrazolium salts to insoluble coloured formazans. The coloured reaction product is proportional to the amount of antigen and can be eluted from cells and measured spectrophotometrically. The application of this technique to a study of the expression of HLA-DR antigens, functionally significant molecules, by mononuclear cells in the cutaneous lesions of leprosy, is described. When a quantitative measure of the HLA-DR expression was related to the area of the granulomata, significant differences in the HLA-DR expression by cells in the infiltrates associated with lesions of tuberculoid and lepromatous leprosy were observed.</p>  a0009-9104