02099nas a2200361   4500000000100000008004100001260001300042653001200055653002100067653002000088653002000108653003500128653001100163653001800174653001800192653001300210653001200223653002600235653001600261653000900277653002100286653001400307653001100321100001400332700001500346700001700361700001400378245016400392300001200556490000700568520114800575022001401723       1985                            d  c1985 Aug10aAnimals10aCandida albicans10aCells, Cultured10aDNA Replication10aFluorescent Antibody Technique10aHumans10aInterleukin-110aInterleukin-210aKinetics10aleprosy10aLymphocyte Activation10aLymphocytes10aMice10aMice, Inbred CBA10aMonocytes10aSpleen1 aVismara D1 aLombardi G1 aPiccolella E1 aColizzi V00aDissociation between interleukin-1 and interleukin-2 production in proliferative response to microbial antigens: restorative effect of exogenous interleukin-2.  a298-3040 v493 a<p>The relationship between the production of interleukin-1 (IL-1) and interleukin-2 (IL-2) after stimulation of human mononuclear cells within an antigenic extract from Candida albicans was analyzed in both responder and nonresponder donors. Culture supernatants from responders contained both IL-1 and IL-2 activity, whereas the supernatants from nonresponders contained only IL-1 and no appreciable IL-2. However, the addition of exogenous IL-2 to nonresponder cultures restored the normal proliferative response. Similar observations were made when cells from mice infected intravenously with high doses of Mycobacterium bovis BCG were cultured; these cells showed a marked impairment of the proliferative response to purified protein derivative. Spleen cells from BCG-induced unresponsive mice failed to produce IL-2 despite the fact that normal IL-1 activity was present in the culture. Again, the addition of exogenous IL-2 fully reversed the proliferative unresponsiveness. Thus, the presence of IL-1 does not necessarily induce production of IL-2, and the proliferative unresponsiveness is therefore due to a primary lack of IL-2.</p>  a0019-9567