01766nas a2200313   4500000000100000008004100001260001300042653001500055653001000070653001200080653001500092653001600107653004400123653001100167653001200178653002600190653001600216653002400232653002500256653001500281100001400296700001300310700001300323245012700336300001000463490000700473520095800480022001401438       1978                            d  c1978 Nov10aAdolescent10aAdult10aAnimals10aArmadillos10aBCG Vaccine10aDose-Response Relationship, Immunologic10aHumans10aleprosy10aLymphocyte Activation10aMiddle Aged10aMycobacterium bovis10aMycobacterium leprae10aTuberculin1 aSmelt A H1 aLiew F Y1 aRees R J00aLymphocyte response of leprosy patients to human-derived and purified armadillo-derived Mycobacterium leprae, BCG and PPD.  a164-90 v343 a<p>The lymphocyte transformation test was applied to compare in vitro lymphocyte responses of tuberculoid (high resistant) and lepromatous (low resistant) leprosy patients to purified Mycobacterium leprae derived from experimentally infected armadillos and crude M. leprae derived from man, as well as to bacille Calmette-Guérin (BCG) and purified protein derivative (PPD). It was found that the purification procedure using enzymic digestion did not affect the immunogenicity of armadillo-derived M. leprae as compared with the crude human-derived preparation, although 2.5-5-fold higher doses of the purified organisms were required to elicitate equivalent lymphocyte responses. The result indicated the suitability of purified armadillo-derived M. leprae as the standard antigen for lymphocytes transformation tests in leprosy. The cross-reactivity studies show a close relationship between PPD and BCG, but not between M. leprae and PPD or BCG.</p>  a0009-9104