01377nas a2200253   4500000000100000008004100001260001300042653001200055653001200067653001600079653000900095653002100104653002500125653001700150653001700167100001500184700001200199245007200211300001100283490000700294050003200301520077600333022001401109       1978                            d  c1978 Jul10aAnimals10aleprosy10aMacrophages10aMice10aMice, Inbred CBA10aMycobacterium leprae10aPhagocytosis10aTime Factors1 aChandi S M1 aJob C K00aThe early cellular response to M. leprae. An ultrastructural study.  a345-570 v50  aInfolep Library - available3 a<p>The ultrastructural changes that develop in mouse peritoneal macrophages from 10 minutes up to 14 weeks after exposure to Mycobacterium leprae are presented. Phagocytosis occurred by a process of engulfment by cytoplasmic processes and incorporation into a phagosome, into which lysosomal enzymes were subsequently introduced. Electron transparent zones (E.T.Z.) were not observed around phagocytosed bacilli in this study, however discrete droplets of lipid-like material appeared in the cytoplasm of macrophages, between 2 and 4 weeks after ingestion of the micro-organisms. Phagosomes with double limiting membranes were observed in macrophages harvested as early as 40 minutes after exposure to M. leprae, contrary to the observations of Evans and Levy (1972).</p>  a0024-1024