01621nas a2200337   4500000000100000008004100001260001700042653001200059653001500071653002400086653001000110653001600120653001100136653002800147653002100175653001200196653000900208100001200217700001600229700001300245700001700258700001400275700001500289700001500304245012200319300001100441490000700452050003200459520077800491022001401269       1986                            d  c1986 Apr-Jun10aAnimals10aAntibodies10aAntigens, Bacterial10aColor10aGlycolipids10aHumans10aImmunoenzyme Techniques10aImmunoglobulin M10aleprosy10aMice1 aKumar S1 aMoudgil K D1 aBand A H1 aNaraynan P R1 aGupta S K1 aSharma A K1 aTalwar G P00aA dot enzyme immunoassay for detection of IgM antibodies against phenolic glycolipid-I in sera from leprosy patients.  a185-900 v58  aInfolep Library - available3 a<p>A visual dipstick dot enzyme immunoassay (EIA) for diagnosis of leprosy is described. The assay is based on detection of IgM antibodies against phenolic glycolipid (PGL-I) in sera from leprosy patients. The antigen (PGL-I or synthetic disaccharide of PGL-I) was dotted on a nitrocellulose pad stuck on a plastic strip (dipstick). Sera were used at a dilution of 1:200. Peroxidase coupled mouse anti-human IgM monoclonal antibodies were used as the conjugate. A positive test gave a blue dot against a white background. The test was highly specific for leprosy, and was quite sensitive for detection of bacilliferous (BL/LL) leprosy. The antigen dotted and preblocked dipsticks stored at room temperature upto 4 months of observation period, were unable in the assay.</p>  a0254-9395