02667nas a2200325   4500000000100000008004100001260001300042653002600055653002400081653002800105653003800133653002100171653001600192653001100208653002100219653002100240653001200261653002500273100001400298700001500312700002100327700001300348700001500361245017700376856006200553300001100615490000700626520169400633022001402327       1986                            d  c1986 May10aAntibodies, Bacterial10aAntigens, Bacterial10aBinding Sites, Antibody10aEnzyme-Linked Immunosorbent Assay10aErythema Nodosum10aGlycolipids10aHumans10aImmunoglobulin G10aImmunoglobulin M10aleprosy10aMycobacterium leprae1 aLevis W R1 aMeeker H C1 aSchuller-Levis G1 aSersen E1 aSchwerer B00aIgM and IgG antibodies to phenolic glycolipid I from Mycobacterium leprae in leprosy: insight into patient monitoring, erythema nodosum leprosum, and bacillary persistence.  uhttp://www.jidonline.org/article/0022-202X(86)90142-9/pdf  a529-340 v863 a<p>Serum IgM and IgG antibodies against Mycobacterium leprae-derived phenolic glycolipid I (PG) were determined in leprosy patients, contacts, and controls by enzyme-linked immunosorbent assay (ELISA). Anti-PG IgM levels increased from the tuberculoid (TT) to the lepromatous (LL) pole of the disease spectrum. There was a positive linear correlation between anti-PG IgM and bacillary index (BI). Patients with erythema nodosum leprosum (ENL) had lower levels of serum anti-PG IgM than non-ENL patients of comparable BI, suggesting that anti-PG IgM is involved in the pathogenesis of ENL. Initial observations indicate that high anti-PG IgM levels in bacillary-negative patients might reflect bacillary persistence. A study of 2 different substrate reagents in the ELISA [2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 0.1 mM H2O2, serum diluted 1:20, and o-phenylenediamine (OPD), 5 mM H2O2, serum diluted 1:300] showed generally good correlation in detection of anti-PG IgM. However the OPD system detected more paucibacillary disease (BT), while the ABTS system detected the significant effect of ENL on the relationship between BI and anti-PG IgM. Anti-PG IgM was clearly dominant over anti-PG IgG. However, certain patients, including several patients who had upgraded from LL and borderline lepromatous leprosy (BL), showed high levels of anti-PG IgG. Since studies have shown that LL patients are selectively deficient in cell-mediated immunity, T-cell products may be required for the IgM to IgG isotype switch. We conclude that anti-PG IgM is useful for monitoring the bacillary load in individual patients and should prove useful for leprosy control strategies.</p>  a0022-202X