01608nas a2200313   4500000000100000008004100001260001300042653002600055653002400081653003800105653001600143653001100159653002100170653001200191653002800203653002500231653002000256100001500276700001400291700001300305700002100318700001600339700001700355245013300372300001000505490000700515520075800522022001401280       1986                            d  c1986 Dec10aAntibodies, Bacterial10aAntigens, Bacterial10aEnzyme-Linked Immunosorbent Assay10aGlycolipids10aHumans10aImmunoglobulin M10aleprosy10aMonitoring, Physiologic10aMycobacterium leprae10aSerologic Tests1 aMeeker H C1 aLevis W R1 aSersen E1 aSchuller-Levis G1 aBrennan P J1 aBuchanan T M00aELISA detection of IgM antibodies against phenolic glycolipid-I in the management of leprosy: a comparison between laboratories.  a530-90 v543 a<p>IgM antibodies to the phenolic glycolipid-I (PGL-I) antigen of Mycobacterium leprae were detected by different ELISA techniques in three laboratories (in New York, Colorado, Seattle, U.S.A.). The agreement on seropositivity and overall correlation between techniques was excellent. A positive linear correlation between the bacterial index (BI) and anti-PGL-I IgM, previously reported by the New York laboratory, was detected by all techniques. The role of erythema nodosum leprosum in decreasing the relationship of BI versus anti-PGL-I IgM was seen by the New York laboratory with sera diluted 1:20 and ABTS substrate solution and by the Colorado laboratory but not by New York with sera at 1:300 and OPD substrate or by the Seattle laboratory.</p>  a0148-916X