01564nas a2200301   4500000000100000008004100001260001300042653001200055653002600067653002300093653002400116653001500140653003800155653001600193653001200209653002500221653001700246653001400263100001500277700001600292700001600308700001700324245010300341300001000444490000700454520078700461022001401248       1986                            d  c1986 Dec10aAnimals10aAntibodies, Bacterial10aAntibody Formation10aAntigens, Bacterial10aArmadillos10aEnzyme-Linked Immunosorbent Assay10aGlycolipids10aleprosy10aMycobacterium leprae10aTime Factors10aXenarthra1 aTruman R W1 aMorales M J1 aShannon E J1 aHastings R C00aEvaluation of monitoring antibodies to PGL-I in armadillos experimentally infected with M. leprae.  a556-90 v543 a<p>An enzyme-linked immunosorbent assay (ELISA) for IgM antibodies to the phenolic glycolipid-I antigen of Mycobacterium leprae was evaluated for efficacy in monitoring armadillos experimentally infected with the bacillus. IgM antibodies were detected in armadillos from 186 days after experimental infection until the animals were sacrificed. The ELISA demonstrated the establishment of infection earlier and more reliably than the histologic methods previously applied. Satisfactory yields of M. leprae from individual armadillos could be predicted 97% of the time, and the technique may be useful in identifying appropriate harvest times or resistance among armadillos. The ELISA seems to be a valuable adjunct for managing experimental infections of M. leprae in armadillos.</p>  a0148-916X