01353nas a2200277   4500000000100000008004100001260001300042653002400055653003800079653001600117653001100133653002600144653001200170653002500182100001600207700001300223700001200236700001700248700001400265700001600279245010700295300001000402490000700412520064200419022001401061       1987                            d  c1987 Jan10aAntigens, Bacterial10aEnzyme-Linked Immunosorbent Assay10aGlycolipids10aHumans10aImmunoelectrophoresis10aleprosy10aMycobacterium leprae1 aKaldany R R1 aMaasho K1 aOhman R1 aReitz-Vick D1 aBritton S1 aLefford M J00aMethods for the detection of a specific Mycobacterium leprae antigen in the urine of leprosy patients.  a37-430 v253 a<p>Two methods for detecting the phenolic glycolipid, PGL-1, a Mycobacterium leprae-specific molecule, in the urine of leprosy patients are described. Both methods rely on the 100-fold preconcentration of the urine, which can be accomplished by a single-step ultrafiltration procedure. The equivalent of approximately 2.5 micrograms of PGL-1/ml was detected in the urine of LL patients with an inhibition ELISA. The second method, a direct dot-blot assay on nitrocellulose paper, was much simpler and more sensitive. As little as 3 ng of antigen was detected by the dot-blot technique. PGL-1 was detected in the urine of LL patients.</p>  a0300-9475