01631nas a2200289   4500000000100000008004100001260001300042653002400055653004100079653001100120653003000131653002100161653001200182653002500194653001600219653001800235100001800253700001100271700001700282700001200299700001500311245009100326300001000417490000700427520089300434022001401327       1986                            d  c1986 Mar10aAntigens, Bacterial10aHistocompatibility Antigens Class II10aHumans10aHypersensitivity, Delayed10aLangerhans Cells10aleprosy10aMycobacterium leprae10aNeutrophils10aT-Lymphocytes1 aNarayanan R B1 aRamu G1 aMalaviya G N1 aSinha S1 aSengupta U00aImmunohistological analysis of skin reaction to My1 derived from Mycobacterium leprae.  a46-510 v543 a<p>A study was made on the in situ characteristics of dermal infiltrates in a 24-hr skin reaction using monoclonal antibodies defining T-cell subsets, Ia-like antigens, Langerhans' cells, My1, and indirect immunofluorescence. The skin reaction was induced by the mycobacterial antigen My1 derived from Mycobacterium leprae. In all, 10 biopsies were studied. The infiltrates were composed of lymphocytes and polymorphonuclear leukocytes. The predominant lymphocytes in the infiltrates were activated T lymphocytes expressing OKT11, Leu3a, OKT8, and Ia-like antigens. The ratio of Leu3a+:OKT8+ cells was 1.2 +/- 0.50. Some of the cells in the infiltrates showed the presence of My1, as seen by the staining with MLO6 monoclonal antibody. No difference was observed in the numbers of OKT6+ epidermal Langerhans' cells in the skin of My1 reaction biopsies and those of normal individuals.</p>  a0148-916X