01615nas a2200337   4500000000100000008004100001260001600042653001200058653002900070653002000099653001100119653001400130653001600144653001900160653001200179653001600191653002600207653001600233653000900249653002200258653002200280653001800302100002100320700001300341700001300354245009200367300001100459490000800470520078500478022001401263       1986                            d  c1986 Oct 1510aAnimals10aAntigen-Presenting Cells10aCell Separation10aFemale10aGranuloma10aGuinea Pigs10aHLA-D Antigens10aleprosy10aLymph Nodes10aLymphocyte Activation10aMacrophages10aMale10aPeritoneal Cavity10aRosette Formation10aT-Lymphocytes1 aMontreewasuwat N1 aCurtis J1 aTurk J L00aAccessory cell function of cells isolated from Mycobacterium leprae-induced granulomas.  a346-540 v1023 a<p>The large cells from Mycobacterium leprae-induced granulomas in guinea pig lymph nodes were separated by Percoll discontinuous density gradient centrifugation and on a fluorescence-activated cell sorter (FACS) using cross-reacting monoclonal antibody to human MHC Class II antigens. Large Percoll-separated cells (83% Class II antigen positive and 52% macrophage-specific antigen positive) and FACS-separated cells are able to act as antigen-presenting cells for T-cell proliferation to PPD. In previous studies, macrophage antigen-positive cells consistently failed to act as accessory cells. This indicates that there is a population of accessory cells which are macrophage antigen negative and MHC Class II antigen positive present in these M. leprae-induced granulomas.</p>  a0008-8749