02068nas a2200301   4500000000100000008004100001260001300042653001200055653002700067653002900094653001800123653001100141653002900152653001200181653003400193653002600227653000900253653002400262653002500286100002200311700001400333700001700347245017200364300001100536490000700547520119800554022001401752       1987                            d  c1987 Feb10aAnimals10aAntibodies, Monoclonal10aAntigen-Antibody Complex10aB-Lymphocytes10aHumans10aImmunoglobulin Idiotypes10aleprosy10aLupus Erythematosus, Systemic10aLymphocyte Activation10aMice10aMice, Inbred BALB C10aTerminology as Topic1 aMackworth-Young C1 aSabbaga J1 aSchwartz R S00aIdiotypic markers of polyclonal B cell activation. Public idiotypes shared by monoclonal antibodies derived from patients with systemic lupus erythematosus or leprosy.  a572-810 v793 a<p>We investigated idiotypic markers of monoclonal antibodies derived from patients with polyclonal B-cell activation. Four monoclonal antibodies with different ligand binding specificities derived from a patient with lepromatous leprosy and three monoclonal anti-DNA antibodies from two patients with SLE were studied. Three new public idiotopes, which were common to monoclonal antibodies from all three patients, were defined by five polyclonal rabbit antiidiotypes, two monoclonal mouse antiidiotopes, and a monoclonal mouse antibody against a synthetic peptide that contains residues of the heavy chain CDR-1 of a monoclonal lupus anti-DNA antibody. The antibody against the synthetic idiotype was found to react with native immunoglobulins in solution. One idiotope was found to be consistently immunogenic in all animals tested. Since the three patients are of different ethnic origins, these shared idiotypes are probably encoded by germline V genes. These genes may be recurrently expressed in states of polyclonal B-cell activation, regardless of etiology. The results suggest that some autoantibodies arise by expansion of a pool of precursors in the normal antibody repertoire.</p>  a0021-9738