01398nas a2200241   4500000000100000008004100001260001700042653002100059653001800080653001900098653002500117653003100142100001500173700001200188700001100200700001200211700001300223245006200236300001100298490000700309520082600316022001401142       1977                            d  c1977 Jul-Sep10aCell-Free System10aCulture Media10aFreeze Etching10aMycobacterium leprae10aMycobacterium lepraemurium1 aNISHIURA M1 aIzumi S1 aMori T1 aTakeo K1 aNonaka T00aFreeze-etching study of human and murine leprosy bacilli.  a248-540 v453 a<p>Morphologic features of the electron transparent zone (ETZ) material around human and murine leprosy bacilli were examined by a freeze-etching technic. The ETZ around human leprosy bacilli is composed of spherical droplets of hydrophobic material. These are always liquid at body temperature and they never show crystalline lamellar structure even at the temperature of liquid nitrogen. The ETZ around murine leprosy bacilli is composed of ribbon-like or membranous crystalline structures. This material is solid and crystalline at the body temperature of mice, and this solid material is the chief cause of the random arrangement of murine leprosy bacilli inside the cytoplasm of murine lepra cells. This crystalline structure has also been observed around murine leprosy bacilli grown on cell-free culture media.</p>  a0148-916X