01535nas a2200289   4500000000100000008004100001260001300042653002700055653001400082653001100096653002800107653001200135653001600147653001500163653002200178653001600200653000900216100001400225700001700239700001700256700001600273245010000289300001000389490000700399520082500406022001401231       1987                            d  c1987 May10aAntibodies, Monoclonal10aGranuloma10aHumans10aImmunoenzyme Techniques10aleprosy10aMacrophages10aPhagocytes10aPulmonary Alveoli10aSarcoidosis10aSkin1 aMunro C S1 aCampbell D A1 aCollings L A1 aPoulter L W00aMonoclonal antibodies distinguish macrophages and epithelioid cells in sarcoidosis and leprosy.  a282-70 v683 a<p>Existing anti-macrophage monoclonal antibodies are unable to differentiate between macrophages and epithelioid cells. In search of more precise reagents, we have applied recently developed antibodies to lesions of sarcoidosis and leprosy. UCHM1 and Leu-M3 stained both granulomas and surrounding histiocytes. However, in lesions with epithelioid granulomas there was a clear distinction between cells identified by RFD9 (epithelioid and giant cells) and RFD7 (macrophages in the surrounding mantle and normal tissue), whereas macrophages in the non-hypersensitivity granulomas of lepromatous leprosy were labelled by both the latter antibodies. In lung biopsies, alveolar macrophages were also labelled by both RFD7 and RFD9. These reagents may be useful for studying pathogenic mechanisms in granuloma formation.</p>  a0009-9104