02463nas a2200373   4500000000100000008004100001260001300042653002700055653004400082653002000126653001400146653001100160653002300171653001700194653001800211653001200229653002600241653001400267653001800281100001500299700002200314700001400336700001300350700001100363700001300374700001200387700001400399245007100413856007800484300001100562490000700573520149500580022001402075       1988                            d  c1988 Feb10aAntibodies, Monoclonal10aAntigens, Differentiation, T-Lymphocyte10aCells, Cultured10aGranuloma10aHumans10aImmunity, Cellular10aInflammation10aInterleukin-210aleprosy10aLymphocyte Activation10aPhenotype10aT-Lymphocytes1 aModlin R L1 aMelancon-Kaplan J1 aYoung S M1 aPirmez C1 aKino H1 aConvit J1 aRea T H1 aBloom B R00aLearning from lesions: patterns of tissue inflammation in leprosy.  uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC279737/pdf/pnas00256-0252.pdf  a1213-70 v853 a<p>The clinical forms of leprosy constitute a spectrum that correlates closely with the degree of cell-mediated immunity. Patients with tuberculoid leprosy develop strong cell-mediated responses and have only a few, localized lesions, whereas patients with multibacillary lepromatous leprosy are specifically unresponsive to antigens of Myobacterium leprae. T cells of the CD4+ subset predominate in tuberculoid lesions, whereas CD8+ cells predominate in lepromatous lesions. Monoclonal antibodies that distinguish subpopulations of CD4+ and CD8+ cells were used to analyze the distribution of T cells infiltrating lesions across the disease spectrum. In lepromatous lesions, T cells of T-suppressor phenotype (9.3-) were the predominant CD8+ cells and suppressor/inducer cells (2H4+, Leu-8+) represented half of the CD4+ subset. In tuberculoid lesions, helper T cells (CD4+4B4+) outnumbered suppressor/inducer T cells by 14:1, compared with a ratio of 1.2:1 in peripheral blood. Analysis of the precursor frequency of antigen-reactive T cells permitted us to estimate that there was a 100-fold enrichment of T cells able to proliferate in response to M. leprae antigens in tuberculoid lesions (2/100), when compared with blood from the same patients. The methods used here to characterize the T-lymphocyte subsets and frequency of antigen-reactive T cells in leprosy may be useful in analyzing immunological reactions occurring in lesions of other inflammatory and autoimmune diseases.</p>  a0027-8424