01917nas a2200301   4500000000100000008004100001260001300042653004100055653001200096653003800108653003800146653001100184653001200195653002900207653002300236653003000259100001200289700001500301700001300316700001900329700001200348245015500360300001100515490000700526050003200533520103600565022001401601       1988                            d  c1988 Apr10aChromatography, High Pressure Liquid10aDapsone10aEnzyme-Linked Immunosorbent Assay10aHemagglutination Inhibition Tests10aHumans10aleprosy10aMetabolic Clearance Rate10aPatient Compliance10aPredictive Value of Tests1 aRaj P P1 aAschhoff M1 aDe Wit M1 aBalakrishnan S1 aLilly L00aCertain aspects of dapsone metabolism in leprosy patients as studied by high performance liquid chromatography (HPLC) and qualitative screening tests.  a215-240 v60  aInfolep Library - available3 a<p>Dapsone (DDS) in urine of 250 leprosy patients collected on surprise visits were screened by simple paper spot, tile tests and sensitive Enzyme linked immunosorbent assay (ELISA) and Haemagglutination inhibition (HI) tests. The urinary DDS concentration as well as DDS/C ratios were also studied. Simultaneously, 50 microliter of blood was collected from each of these patients and its dapsone content was estimated by HPLC. Urine samples with means of 25 to 30 micrograms/ml DDS and 55-64 micrograms/mg DDS/C ratios were found to give positive tests by any of the above screening procedures, while their mean blood DDS concentration was found to be 0.91 microgram/ml. The corresponding values for those specimens giving negative tests were 3.8 to 5.7 micrograms DDS per ml and 9 to 13 micrograms/mg DDS/C ratio. The blood DDS concentration in this group was ranging from 0.16 to 0.18 micrograms/ml. The findings are discussed in relation to their metabolic significance and their application in a leprosy control programme.</p>  a0254-9395