01378nas a2200289   4500000000100000008004100001260001300042653002400055653003800079653001600117653001800133653001100151653002000162653001200182653002100194653002100215653002500236653002000261100001200281700001700293700001600310245011000326300001100436490000700447520062000454022001401074       1986                            d  c1986 Jan10aAntigens, Bacterial10aEnzyme-Linked Immunosorbent Assay10aGlycolipids10aGlycoproteins10aHumans10aImmunodiffusion10aleprosy10aMethylglucosides10aMethylglycosides10aMycobacterium leprae10aSerologic Tests1 aCho S N1 aChatterjee D1 aBrennan P J00aA simplified serological test for leprosy based on a 3,6-di-O-methylglucose-containing synthetic antigen.  a167-720 v353 a<p>The recent advent of synthetic antigens containing the Mycobacterium leprae-specific epitope, 3,6-di-O-methyl-beta-D-glucopyranoside, has allowed the development of simple specific serological tests for leprosy. The incorporation of one such product, 8-carbonyloctyl O-[4-O-(3,6-di-O-methyl-beta-D-glucopyranosyl)-alpha-L- rhamnopyranoside]-BSA, into a simple "spot" test, diffusion-in-gel enzyme-linked immunosorbent assay (ELISA), allowed an over 90% detection rate of untreated lepromatous leprosy, and the results showed good concordance with conventional ELISA based on the native phenolic glycolipid I.</p>  a0002-9637