02385nas a2200277   4500000000100000008004100001260001600042653002400058653001400082653003800096653001600134653004100150653001100191653002400202653001200226653002500238653003100263100001500294700001400309700001600323245012000339300001300459490000800472520161300480022001402093       1986                            d  c1986 Sep 1510aAntigens, Bacterial10aArabinose10aEnzyme-Linked Immunosorbent Assay10aFatty Acids10aGas Chromatography-Mass Spectrometry10aHumans10aLipopolysaccharides10aMannose10aMycobacterium leprae10aMycobacterium tuberculosis1 aHunter S W1 aGaylord H1 aBrennan P J00aStructure and antigenicity of the phosphorylated lipopolysaccharide antigens from the leprosy and tubercle bacilli.  a12345-510 v2613 a<p>A family of major arabinose- and mannose-containing phosphorylated lipopolysaccharides was isolated from Mycobacterium leprae and Mycobacterium tuberculosis. The only antigenic member of the family, lipoarabinomannan (LAM)-B, was purified by anion exchange and gel filtration chromatography in detergent and recovered in large quantities (15 mg/g of bacteria). It yielded a broad diffuse band on polyacrylamide gel electrophoresis but appeared homogeneous by this criterion and gel filtration. Besides arabinose and mannose, it contained glycerol and a polyol phosphate and was acylated by lactate, succinate, palmitate, and 10-methyloctadecanoate. The phosphate was released by alkalinolysis and identified by thin layer chromatography and gas chromatography-mass spectrometry as myoinositol 1-phosphate. Thus, the group-specific "arabinomannan" of the genus Mycobacterium in the native state is acylated, contains the substituents of phosphatidylinositol, and is apparently membrane associated. LAM-B is one of the dominant immunogens of the leprosy bacillus reacting readily with antibodies from lepromatous leprosy patients and monoclonal antibodies in plate and nitrocellulose enzyme-linked immunosorbent assay and on electrophoretic immunoblots. It is immunologically cross-reactive with a like product from M. tuberculosis. LAM-B is clearly the pervasive "glycoprotein" antigen of the leprosy bacillus and may be the long sought lipoteichoic acid-like polymer of Mycobacterium with a role in cell wall physiology, macrophage recognition, and perhaps an involvement in cross-protective immunity.</p>  a0021-9258