01992nas a2200325   4500000000100000008004100001260001300042653002600055653002400081653003800105653001600143653001100159653002100170653002100191653001200212653002400224653002500248653003100273100001400304700001500318700002100333700001300354700001600367700001200383245009000395300001000485490000800495520114900503022001401652       1987                            d  c1987 Nov10aAntibodies, Bacterial10aAntigens, Bacterial10aEnzyme-Linked Immunosorbent Assay10aGlycolipids10aHumans10aImmunoglobulin G10aImmunoglobulin M10aleprosy10aLipopolysaccharides10aMycobacterium leprae10aMycobacterium tuberculosis1 aLevis W R1 aMeeker H C1 aSchuller-Levis G1 aSersen E1 aBrennan P J1 aFried P00aMycobacterial carbohydrate antigens for serological testing of patients with leprosy.  a763-90 v1563 a<p>To determine whether quantitation of antibodies to mycobacterial carbohydrate determinants would be valuable in serodiagnosis and monitoring of leprosy, we tested serum IgM antibody to Mycobacterium leprae phenolic glycolipid I and IgM and IgG antibodies to Mycobacterium tuberculosis and M. leprae lipoarabinomannan (LAM) by enzyme-linked immunosorbent assay. Seventy-one percent of patients with paucibacillary disease and 85.5% of patients with multibacillary disease were positive for at least one of the three antibodies. The 15% of antibody-negative patients with multibacillary disease were mostly long-term-treated patients, with inactive disease by biopsy. There was excellent agreement between M. tuberculosis LAM and M. leprae LAM in detection of antibodies. Bacillary index and levels of both IgG and IgM antibodies to LAM were positively correlated when all patients were analyzed. When patients with a history of erythema nodosum leprosum (ENL) were analyzed separately, there was no correlation between IgM or IgG antibody to LAM and bacillary index, a result suggesting a possible role for LAM in the pathogenesis of ENL.</p>  a0022-1899