02485nas a2200421   4500000000100000008004100001260001600042653002400058653001200082653002900094653002400123653001600147653000800163653002400171653001900195653002000214653002000234653001100254653002400265653001200289653000900301653002800310653002500338653003200363653001800395100001600413700001400429700001700443700001300460700002000473700001600493700001600509245017000525300001200695490000800707520133400715022001402049       1988                            d  c1988 Apr 0110aAmino Acid Sequence10aAnimals10aAntigen-Presenting Cells10aAntigens, Bacterial10aClone Cells10aDNA10aGenes, MHC Class II10aHLA-D Antigens10aHLA-DR Antigens10aHLA-DR2 Antigen10aHumans10aL Cells (Cell Line)10aleprosy10aMice10aMolecular Sequence Data10aMycobacterium leprae10aRecombinant Fusion Proteins10aT-Lymphocytes1 aWilkinson D1 aVries R R1 aMadrigal J A1 aLock C B1 aMorgenstern J P1 aTrowsdale J1 aAltmann D M00aAnalysis of HLA-DR glycoproteins by DNA-mediated gene transfer. Definition of DR2 beta gene products and antigen presentation to T cell clones from leprosy patients.  a1442-580 v1673 a<p>We have used DNA-mediated gene transfer to express HLA class II molecules in mouse L cells for serological, biochemical, and functional analysis. cDNA clones encoding the DR2 beta a and DR2 beta b products of the DR2Dw2 haplotype were subcloned into a mouse Moloney leukemia virus-based expression vector (pJ4) and transfected separately into mouse L cells together with a HLA-DR alpha/pJ4 construct. These transfectants have allowed differential analysis of the two DR2 beta products in a manner normally prohibited by the concomitant expression seen in B cells. Two-dimensional SDS-PAGE analysis of the transfectants defines the more acidic beta chain as the product of the DR2 beta a sequence, and the more basic chain as the product of the DR2 beta b sequence. The LDR2a transfectants present antigen efficiently to M.leprae-specific T cell clones and are capable of presenting synthetic peptide, 65-kD recombinant mycobacterial antigen and M.leprae. Of the DR2Dw2-restricted T cell clones we have tested, all use the DR2 beta a chain as their restriction element. Inhibition studies with mAbs demonstrate the dependence of presentation by the transfectant on class II and CD4, while mAbs against LFA-1, which substantially inhibit presentation by B-lymphoblastoid cell lines, do not inhibit transfectant presentation.</p>  a0022-1007