01716nas a2200313   4500000000100000008004100001260000900042653001200051653001800063653001500081653002000096653001100116653001400127653001600141653001200157653001600169653002600185653001600211653002500227100001500252700001500267700001300282700001300295245011600308300001000424490000700434520094700441022001401388       1988                            d  c198810aAnimals10aB-Lymphocytes10aCell Count10aDendritic Cells10aFemale10aGranuloma10aGuinea Pigs10aleprosy10aLymph Nodes10aLymphocyte Activation10aMacrophages10aMycobacterium leprae1 aVerghese S1 aHealey D G1 aCurtis J1 aTurk J L00aAccessory cell function of dendritic cells from lymph nodes containing Mycobacterium leprae induced granulomas.  a392-90 v873 a<p>Dendritic cells were enriched from guinea pig auricular lymph nodes containing Mycobacterium leprae induced granulomas by immunomagnetic depletion of other cells. These cells were strongly positive for major histocompatibility complex class II antigens and labelled with an antidendritic cell monoclonal antibody, but not with an antimacrophage antibody. Interdigitating dendritic cells were identified in the granulomatous lymph node by staining with the antidendritic cell antibody and by transmission electron microscopy. When cultured in vitro with purified T lymphocytes, these cells acted as accessory cells for both purified protein derivative and concanavalin A induced proliferation. Although previous studies have shown that macrophages from these lymph nodes do not act as accessory cells, the present results indicate that dendritic cells from M. leprae granuloma containing lymph nodes may act as antigen-presenting cells.</p>  a0020-5915