01245nas a2200229   4500000000100000008004100001260001300042653002700055653002400082653004000106653003800146653001100184653001200195653002500207100001200232700001600244245008300260300001100343490000800354520063900362022001401001       1988                            d  c1988 Jun10aAntibodies, Monoclonal10aAntigens, Bacterial10aElectrophoresis, Polyacrylamide Gel10aEnzyme-Linked Immunosorbent Assay10aHumans10aleprosy10aMycobacterium leprae1 aWit M Y1 aKlatser P R00aPurification and characterization of a 36 kDa antigen of Mycobacterium leprae.  a1541-80 v1343 a<p>A 36 kDa antigen of Mycobacterium leprae was purified by phenol biphasic partition followed by preparative SDS-PAGE. The purified antigen appeared as a single band in SDS-PAGE and eluted as a single peak in ion-exchange chromatography. The antigen comprised epitopes which were cross-reactive with M. tuberculosis, as well as a species-specific epitope (recognized by MAb F47-9). Different treatments of the 36 kDa antigen suggested it to be largely protein in nature; the amino acid composition of 81% of the antigen was determined. A majority of sera from leprosy patients contained antibodies recognizing the 36 kDa antigen.</p>  a0022-1287