01353nas a2200301   4500000000100000008004100001260001700042653001200059653003100071653001000102653001100112653002800123653003200151653002100183653002000204653002700224653001200251653001200263653002200275653001300297100001300310700001400323245008000337300001000417490000700427520060300434022001401037       1988                            d  c1988 Aug-Oct10aAnimals10aAntigen-Antibody Reactions10aGoats10aHumans10aImmunoenzyme Techniques10aImmunoglobulin Fc Fragments10aImmunoglobulin G10aKidney Diseases10aKidney Transplantation10aleprosy10aRabbits10aRheumatoid Factor10aSyphilis1 aMiyata M1 aMilgrom F00aEnzyme immunoassay for IgG rheumatoid factor combining with homologous IgG.  a561-50 v173 a<p>An enzyme immunoassay (EIA) was developed to detect, in human sera, IgG rheumatoid factor (RF) combining with human IgG. Wells of the EIA plate were coated with the Fc fragment of human IgG. Binding of RF was detected by goat antibodies to F(ab')2 of human IgG followed by rabbit anti-goat IgG conjugated with alkaline phosphatase and finally by the substrate. Using this procedure, RF was detected in 35%-85% of various pathological human sera, but only in 7% of normal human sera. An analogous procedure was also described for detection, in rabbit sera, of an RF combining with rabbit IgG.</p>  a0882-0139