02204nas a2200361   4500000000100000008004100001260001300042653001000055653001200065653002700077653002400104653002200128653001100150653001100161653002100172653001200193653000900205653000900214653002500223653002100248100001700269700001500286700001800301700001700319700001400336700001500350245007800365856007800443300001100521490000700532520128900539022001401828       1988                            d  c1988 Dec10aAdult10aAnimals10aAntibodies, Monoclonal10aAntigens, Bacterial10aBlotting, Western10aFemale10aHumans10aImmunoglobulin G10aleprosy10aMale10aMice10aMycobacterium leprae10aReference Values1 aVega-Lopez F1 aStoker N G1 aLocniskar M F1 aDockrell H M1 aGrant K A1 aMcAdam K P00aRecognition of mycobacterial antigens by sera from patients with leprosy.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC266928/pdf/jcm00084-0022.pdf  a2474-90 v263 a<p>Mycobacterium leprae sonic extracts prepared from armadillo-derived bacteria were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (immunoblotting) procedures and probed with serum or plasma samples from 20 patients with lepromatous leprosy and 14 healthy endemic controls. Five proteins of 33, 25, 18, 15, and 12 kilodaltons (kDa) were frequently recognized; the 33- and 15-kDa proteins were, respectively, recognized with high intensity by 16 and 13 of the 20 samples from patients with leprosy, whereas only one healthy donor had antibodies that recognized the 15-kDa protein. By the use of M. leprae-specific murine monoclonal antibodies it was demonstrated that the 33-, 25-, and 15-kDa antigens were different from those bound by the available murine monoclonal antibodies. The 18- and 12-kDa proteins detected had molecular masses similar to those detected by the corresponding murine monoclonal antibodies. The serum and plasma samples from patients with leprosy were also used to probe Western blots of a soluble extract of M. tuberculosis. They recognized, among others, antigens with molecular weights similar to those detected in the M. leprae antigenic preparations, although with less intensity and at a lower frequency.</p>  a0095-1137