01784nas a2200361   4500000000100000008004100001260001300042653001000055653001900065653003300084653001100117653002300128653001200151653001300163653002500176653001900201653003000220653001300250653002700263653001300290100001600303700001400319700001800333700001400351700001500365700001600380245010700396856006100503300001000564490000700574520082700581022001401408       2000                            d  c2000 Sep10aCodon10aDNA, Bacterial10aDNA-Directed RNA Polymerases10aHumans10aLeprostatic Agents10aleprosy10aMutation10aMycobacterium leprae10aPlant Proteins10apolymerase chain reaction10aRifampin10aSequence Analysis, DNA10aThailand1 aRamasoota P1 aWongwit W1 aSampunachot P1 aUnnarat K1 aNgamying M1 aSvenson S B00aMultiple mutations in the rpoB gene of Mycobacterium leprae strains from leprosy patients in Thailand.  uhttp://www.tm.mahidol.ac.th/seameo/2000_31_3/15-2578.pdf  a493-70 v313 a<p>A new finding is reported of multiple mutations in the rpoB gene of 9 Mycobacterium leprae strains from leprosy patients in Thailand, who did not respond to therapy even when rifampicin, the main drug in multi-drug therapy was used. By means of sequence analysis of 9 Thai M. leprae strains, various mutations in 289 bps of the rpoB gene revealed forms of mutation never before described, such as multiple mutations (ie, mutation at two, three, six, seven, eight and nine positions in the rpoB gene), most of which were point-mutation substitutions (a few of which were silent), and some insertions. This investigation demonstrates that mutation in the rpoB gene of M. leprae strains from Thailand involves more variety than previously reported for rpoB mutation patterns in rifampicin resistance M. leprae strains.</p>  a0125-1562