01864nas a2200265   4500000000100000008004100001260001300042653002000055653002000075653001100095653003000106653001200136653001200148653002600160653001800186100001500204700001500219700001400234700001300248245021900261300001000480490000800490520108600498022001401584       1977                            d  c1977 Jan10aCell Separation10aElectrophoresis10aHumans10aImmune Adherence Reaction10aLectins10aleprosy10aLymphocyte Activation10aT-Lymphocytes1 aTalwar G P1 aHanjan S N1 aMehra V L1 aKidwai Z00aLack of interaction of circulating T cells with phytohemagglutinin in bacillary positive untreated lepromatous leprosy patients--identification of subpopulation of lymphocytes by shifts in electrophoretic mobility.  a242-70 v1183 a<p>Incubation of human peripheral blood lymphocytes from normal healthy subjects with phytohamagglutinin (PHA), causes the reduction of the surface charge of a subpopulation of T cells by 1363 +/- 242 e.s.u./cm2. The affected subpopulation was predominantly the high charge-bearing cells identifiable with early (10 min) rosette-forming cells with sheep erythrocytes. Purified lymphocytes obtained from untreated bacillary-positive, lepromatous leprosy patients contained high charge-bearing T lymphocyte subpopulation. However, incubation with PHA did not result in the shift of electrophoretic mobility of these cells, suggesting the absence of interacting sites for the mitogen on the surface of these cells. The absence of mitogen-interacting sites is not an inherent trait of leprosy patients; the surface charge of lymphocytes from Dapsone-treated bacillary-negative subjects was reduced upon incubation with PHA. A close correlation was found between the number of cells whose charge alters on incubation with PHA and the transformation index obtained with this mitogen.</p>  a0022-1767