01773nas a2200361   4500000000100000008004100001260001300042653002700055653003000082653001800112653002500130653001400155653001100169653001700180653001200197653001600209653001400225653001600239653003300255653001800288100001500306700002100321700001400342700001300356700001800369700001400387700001500401245013800416300001100554490000600565520082600571022001401397       1988                            d  c1988 Apr10aAntibodies, Monoclonal10aAntigens, Differentiation10aCD11 Antigens10aCell Differentiation10aCell Line10aHumans10aImmunization10aleprosy10aMacrophages10aMonocytes10aSarcoidosis10aTetradecanoylphorbol Acetate10aToxoplasmosis1 aCabañas C1 aSanchez-Madrid F1 aAcevedo A1 aBellon T1 aFERNANDEZ J M1 aLarraga V1 aBernabeu C00aCharacterization of a CD11c-reactive monoclonal antibody (HC1/1) obtained by immunizing with phorbol ester differentiated U937 cells.  a167-760 v73 a<p>A mouse monoclonal antibody (HC1/1) specific for a differentiation marker of human monocytes and granulocytes has been generated by using as immunogen the monocytic cell line U937 differentiated with phorbol esters. This differentiation antigen has been characterized as the p150/95 member of the CD11 family of glycoproteins by cellular distribution studies, immunoprecipitation and competition experiments with MAb 3.9 specific for the CD11c antigen. Immuno-alkaline phosphatase staining of normal tissue sections with the HC1/1 MAb demonstrated that the CD11c antigen is a useful macrophage marker. Furthermore, the MAb HC1/1 stains specifically populations of macrophages on skin and lymph node sections from different pathologies such as Sarcoidosis, Granuloma annulare, Lepromatous leprosy and Toxoplasmosis.</p>  a0272-457X