02200nas a2200361   4500000000100000008004100001260001300042653002700055653003100082653001400113653001100127653002300138653002800161653002100189653002500210653001500235653002400250653002500274653002900299653003400328653003000362100001200392700001200404700001400416700001500430700001800445700001300463245013400476300001100610490000700621520119600628022001401824       1988                            d  c1988 May10aAntibodies, Monoclonal10aCD4-Positive T-Lymphocytes10aGranuloma10aHumans10aImmunity, Cellular10aImmunoenzyme Techniques10aLangerhans Cells10aLeprosy, lepromatous10aLeukocytes10aMycobacterium bovis10aMycobacterium leprae10aT-Lymphocytes, Cytotoxic10aT-Lymphocytes, Helper-Inducer10aT-Lymphocytes, Regulatory1 aGross A1 aWeiss E1 aTapia F J1 aAranzazu N1 aGallinoto M E1 aConvit J00aLeukocyte subsets in the granulomatous response produced after inoculation with Mycobacterium leprae-BCG in lepromatous patients.  a608-120 v383 a<p>Leukocyte subsets present in the granulomatous response produced after the inoculation of a mixture of Mycobacterium leprae and BCG in lepromatous leprosy patients were characterized in situ using monoclonal antibodies and an immunoperoxidase technique. The granuloma produced after M. leprae-BCG inoculation showed a distribution pattern similar to tuberculoid granulomas. T lymphocytes bearing the CD8 phenotype (T cytotoxic/suppressor) were sequestered to the periphery of the epithelioid tubercles and T helper-inducer CD4+ lymphocytes were distributed throughout the infiltrate. Langerhans cells CD1+ were increased in the epidermis, and in dermis they were localized mainly in the mantle surrounding the granuloma. Most of the dermal infiltrate produced after the inoculation or M. leprae-BCG expresses the HLA-DR antigen. Similarly, most keratinocytes were also positive to this MHC antigen. The granulomatous response to BCG was similar to the inoculation of a mixture of M. leprae-BCG, however acid-fast bacilla were still present. The inoculation of M. leprae produced a macrophage granuloma with no clearing of the bacilla which resembles the lepromatous leprosy granuloma.</p>  a0002-9637