02454nas a2200421   4500000000100000008004100001260001300042653001500055653001000070653000900080653002500089653001000114653003000124653002100154653001100175653001100186653001600197653001200213653002500225653001600250653000900266653001600275100001600291700001600307700001800323700001400341700001500355700001100370700002000381700001800401700001500419245004700434856009000481300001000571490000700581520143000588022001402018       1989                            d  c1989 Jun10aAdolescent10aAdult10aAged10aAntilymphocyte Serum10aChild10aCytotoxicity, Immunologic10aErythema Nodosum10aFemale10aHumans10aIsoantigens10aleprosy10aLeprosy, lepromatous10aLymphocytes10aMale10aMiddle Aged1 aRasheed F N1 aLocniskar M1 aMcCloskey D J1 aHasan R S1 aChiang T J1 aRose P1 aDe Soldenhoff R1 aFestenstein H1 aMcAdam K P00aSerum lymphocytotoxic activity in leprosy.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1541899/pdf/clinexpimmunol00087-0077.pdf  a391-70 v763 a<p>Sera from 167 patients across the spectrum of leprosy and 46 endemic controls were screened for lymphocytotoxic activity (LCA). The Terasaki microdroplet lymphocytotoxicity assay was performed at 37 degrees C and 15 degrees C to test sera for LCA against a panel of lymphocytes from 50 donors which represented most known HLA-ABC antigens. Raised complement-dependent LCA at 15 degrees C was seen in leprosy patients with histories of erythema nodosum leprosum (ENL) or reversal/Type I (I) reactions. Eighty-six per cent of lepromatous (LL) patients with a history of ENL (n = 21, P less than 0.001), 83% of borderline lepromatous (BL) and 88% of borderline tuberculoid patients (BT) with a history of Type I reactions (n = 12, P less than 0.01 and n = 24, P less than 0.001 respectively) had LCA compared to 39% of endemic controls (n = 46). LCA was attributed to IgM on the basis of reduced activity when serum was treated with both dithiothreitol or absorbed with antiserum for IgM. Removal of immune complexes and rheumatoid factor did not influence LCA. LCA-positive sera reacted similarly with allogeneic lymphocytes from either healthy donors or leprosy patients. Moreover LCA-positive sera reacted with autologous lymphocytes. Specificities for HLA-ABC antigens were not identified. The potential role of these autoantibodies, manifested in leprosy patients with hypersensitivity reactions remains speculative.</p>  a0009-9104