01602nas a2200301   4500000000100000008004100001260001300042653001200055653001100067653001400078653001600092653004100108653002500149653002500174653002900199653002800228653001800256100001500274700001300289700002100302700001300323700001300336245020900349300001100558490000700569520071000576022001401286       1989                            d  c1989 Aug10aAnimals10aFemale10aGranuloma10aGuinea Pigs10aHistocompatibility Antigens Class II10aImmunohistochemistry10aMicroscopy, Electron10aMycobacterium Infections10aNervous System Diseases10aSciatic Nerve1 aCowley S A1 aButter C1 aGschmeissner S E1 aCurtis J1 aTurk J L00aAn immunoelectronmicroscopical study of the expression of major histocompatibility complex (MHC) class II antigens in guinea pig sciatic nerves following induction of intraneural mycobacterial granulomas.  a223-310 v233 a<p>A guinea pig model of nerve damage in leprosy has been used to investigate the expression of major histocompatibility complex (MHC) class II antigens in granulomatous lesions in nerves. Using an immunoelectronmicroscopical technique, infiltrating mononuclear cells and endoneural fibroblast-like cells are shown to be class II-positive in the experimental neural lesions. Schwann cells are not class II-positive under these conditions, although at the light microscope level Schwann cell-like cells appear to be positively stained. This illustrates the value of immunoelectronmicroscopy in the investigation of cell surface proteins in situ as compared with conventional light immunohistochemistry.</p>  a0165-5728