@article{98011,
  keywords = {Virology, Genetics, Molecular Biology, Immunology, Microbiology, Parasitology},
  author = {Dallmann-Sauer M and Xu YZ and da Costa ALF and Tao S and Gomes TA and Prata RBDS and Correa-Macedo W and Manry J and Alcaïs A and Abel L and Cobat A and Fava VM and Pinheiro RO and Lara FA and Probst CM and Mira MT and Schurr E},
  editor = {Hawn TR},
  title = {Allele-dependent interaction of LRRK2 and NOD2 in leprosy},
  abstract = {<p>Leprosy, caused by Mycobacterium leprae, rarely affects children younger than 5 years. Here, we studied a multiplex leprosy family that included monozygotic twins aged 22 months suffering from paucibacillary leprosy. Whole genome sequencing identified three amino acid mutations previously associated with Crohn’s disease and Parkinson’s disease as candidate variants for early onset leprosy: LRRK2 N551K, R1398H and NOD2 R702W. In genome-edited macrophages, we demonstrated that cells expressing the LRRK2 mutations displayed reduced apoptosis activity following mycobacterial challenge independently of NOD2. However, employing co-immunoprecipitation and confocal microscopy we showed that LRRK2 and NOD2 proteins interacted in RAW cells and monocyte-derived macrophages, and that this interaction was substantially reduced for the NOD2 R702W mutation. Moreover, we observed a joint effect of LRRK2 and NOD2 variants on Bacillus Calmette-Guérin (BCG)-induced respiratory burst, NF-κB activation and cytokine/chemokine secretion with a strong impact for the genotypes found in the twins consistent with a role of the identified mutations in the development of early onset leprosy.</p>
},
  year = {2023},
  journal = {PLOS Pathogens},
  volume = {19},
  pages = {1-32},
  publisher = {Public Library of Science (PLoS)},
  issn = {1553-7374},
  url = {https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1011260&type=printable},
  doi = {10.1371/journal.ppat.1011260},
  language = {Eng},
}