@article{9782,
  keywords = {Antigens, Bacterial, Disaccharides, Enzyme-Linked Immunosorbent Assay, Glycolipids, Humans, leprosy, Mycobacterium leprae, Serum Albumin, Bovine, Tuberculosis},
  author = {Wu Q X},
  title = {[Studies on the serological activity of ND-O-BSA and its application in serodiagnosis of leprosy].},
  abstract = {<p>In this article, optimum conditions of ND-O-BSA-ELISA were determined and comparisons made of the serologic activity of 3 different types of antigens (WML, PGI, ND-O-BSA) in leprosy (151) and tuberculosis (20) patients and normal controls (42). The results indicated that: 1) optimum conditions for ND-O-BSA-ELISA were ND-O-BSA and OPD concentration of 0.1 and 400 micrograms/ml respectively with HRP-IgM and test sera dilutions of 1:1000 and 1:200; and 2) serological activity showed highly significant positive correlations between 3 types of antigens (WML vs PGI: r = 0.88, P less than 0.0005; PGI vs ND-O-BSA: r = 0.86, P less than 0.0005), all of which were highly sensitive and specific for leprosy serodiagnosis with positive rates of 100% (PGI) and 97.4% (WML, ND-O-BSA) in leprosy patients. None were positive in normal controls, but crossreactivity of different degree were present in tuberculosis patients. The authors suggest that PGI-ELISA and ND-O-BSA-ELISA be used in combination in the evaluation of clinical therapeutic efficacy and determination of disease stages, as well as in epidemiologic survey of subclinical leprosy infection in "high-risk" areas.</p>},
  year = {1989},
  journal = {Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae},
  volume = {11},
  pages = {220-4},
  month = {1989 Jun},
  issn = {1000-503X},
  language = {chi},
}