@article{96233,
  keywords = {Mycobacterium leprae, infection, tick-derived cells, ticks, transmission},
  author = {Tongluan N and Shelton L and Collins HJ and Ingraffia P and McCormick G and Pena M and Sharma R and Lahiri R and Adams L and Truman RW and Macaluso K},
  title = { Infection in Ticks and Tick-Derived Cells.},
  abstract = {<p>Leprosy is a zoonosis in the southern United States involving humans and wild armadillos. The majority of patients presenting with zoonotic strains of note extensive outdoor activity but only rarely report any history of direct contact with wild armadillos. Whether is transmitted to new vertebrate hosts through the environment independently or with the aid of other organisms, e.g., arthropod vectors, is a fundamental question in leprosy transmission. The objectives of this study were to assess the potential for ticks to transmit and to test if viable can be maintained in tick-derived cells. To evaluate tick transmission, nymphal ticks were injected with isolated Infection and transmission were assessed by qPCR. Ticks infected as nymphs harbored through vertical transmission events (nymph to adult and adult to progeny); and, horizontal transmission of to a vertebrate host was observed. DNA was detected in multiple tick life cycle stages. Likewise, freshly isolated (Thai-53) was used to infect a tick-derived cell line, and enumeration and bacterial viability were assessed at individual time points for up to 49 days. Evaluations of the viability of long-term cultured (Thai-53 and Br4923) were also assessed in a mouse model. Tick-derived cells were able to maintain viable over the 49-day course of infection and remained infectious within tick cells for at least 300 days. The results of this study suggest that ticks themselves might serve as a vector for the transmission of and that tick cells are suitable for maintenance of viable for an extended period of time.</p>
},
  year = {2021},
  journal = {Frontiers in microbiology},
  volume = {12},
  pages = {761420},
  month = {01/2021},
  issn = {1664-302X},
  url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578725/pdf/fmicb-12-761420.pdf},
  doi = {10.3389/fmicb.2021.761420},
  language = {eng},
}