@article{8621,
  keywords = {Antibodies, Bacterial, Antigens, Bacterial, Brazil, Case-Control Studies, Chile, Endemic Diseases, Enzyme-Linked Immunosorbent Assay, Glycolipids, Humans, Immunoglobulin M, leprosy, Mycobacterium leprae, ROC Curve, Reagent Kits, Diagnostic, Reproducibility of Results},
  author = {Silva RC and Lyon S and Araos R and Lyon AC and Faria Grossi MA and Lyon SH and Penido RA and Bührer-Sékula S and Antunes CMF},
  title = {The result patterns of ML Flow and ELISA (PGL-I) serologic tests in leprosy-endemic and non-endemic areas.},
  abstract = {<p>ML Flow and anti-PGL-I ELISA are serological tests that detect IgM antibodies against the phenolic glycolipid I (PGL-I), specific to Mycobacterium leprae. To evaluate the outcomes of ML Flow and ELISA (PGL-I) serological tests in leprosy-endemic areas in comparison to non-endemic ones, a total of 351 volunteers from Brazil and Chile were examined, including leprosy patients, healthy controls and others affected by other infectious or non-infectious diseases that are common differential diagnoses for leprosy. The ELISA cut-off point was established using the ROC Curve method (>or= 0.157). In endemic areas, 70% of leprosy patients present positive ML Flow results and 53.3% were ELISA-positive. In non-endemic areas, ML Flow was negative in all the subjects tested and ELISA was positive in 4 volunteers. ML Flow is faster and more easily performed and, therefore, a more adequate test for use in basic, primary-level health care centers. ELISA requires trained personnel, in addition to a more complex laboratory infrastructure.</p>},
  year = {2008},
  journal = {Revista da Sociedade Brasileira de Medicina Tropical},
  volume = {41 Suppl 2},
  pages = {19-22},
  month = {2008},
  issn = {1678-9849},
  doi = {10.1590/s0037-86822008000700005},
  language = {eng},
}