@article{8164,
  keywords = {Bacterial Proteins, Developing countries, Drug Resistance, Bacterial, Gene Expression Regulation, Bacterial, Humans, Leprostatic Agents, leprosy, Mutation, Myanmar, Mycobacterium leprae, Oligonucleotide Array Sequence Analysis, Philippines, Prevalence},
  author = {Matsuoka M and Aye KS and Kyaw K and Tan EV and Balagon MVF and Saunderson P and Gelber R and Makino M and Nakajima C and Suzuki Y},
  title = {A novel method for simple detection of mutations conferring drug resistance in Mycobacterium leprae, based on a DNA microarray, and its applicability in developing countries.},
  abstract = {<p>A simple method to detect mutations in the genome of Mycobacterium leprae that confer resistance to key drugs for leprosy was exploited on the basis of a reverse hybridization system. A series of oligonucleotide probes corresponding to each mutation in the folP1, rpoB and gyrA genes for dapsone, rifampicin and ofloxacin resistance, respectively, were selected and fixed on a glass slide as capture probes, to develop a DNA microarray termed the leprosy drug susceptibility-DNA microarray (LDS-DA). Mutations in clinical isolates of M. leprae were successfully identified by the LDS-DA. Feasibility studies were conducted to evaluate the performance of the LDS-DA in two developing countries, Myanmar and the Philippines. The high concordance of the results obtained by this method with the results of nucleotide sequencing strongly supports the applicability of the LDS-DA as a drug susceptibility test in place of sequencing, a time-consuming and costly procedure. This is a rapid and simple method for the simultaneous susceptibility testing of three front-line drugs for leprosy, and solves the problems of previously reported methods.</p>},
  year = {2008},
  journal = {Journal of medical microbiology},
  volume = {57},
  pages = {1213-9},
  month = {2008 Oct},
  issn = {0022-2615},
  doi = {10.1099/jmm.0.2008/002600-0},
  language = {eng},
}