@article{7251,
  keywords = {Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antigens, Bacterial, Base Sequence, Cloning, Molecular, Epitopes, Guinea Pigs, Hypersensitivity, Delayed, Molecular Sequence Data, Molecular Weight, Mycobacterium leprae, Proline, Recombinant Proteins, Restriction Mapping, Skin Tests},
  author = {Thole J E and Stabel L F and Suykerbuyk M E and Wit M Y and Klatser P R and Kolk A H and Hartskeerl R A},
  title = {A major immunogenic 36,000-molecular-weight antigen from Mycobacterium leprae contains an immunoreactive region of proline-rich repeats.},
  abstract = {<p>The 36,000-molecular-weight antigen (36K antigen) of Mycobacterium leprae is a major immunogenic protein carrying common and specific antigenic determinants recognized by antibodies and T cells in leprosy patients. Recombinant DNA clones containing the complete gene coding for the 36 K antigen, designated in this paper as PRA, were isolated from both lambda gt11 and cosmid libraries of the M. leprae genome. The DNA sequence of the pra gene coded for a polypeptide of 249 amino acids with a predicted molecular mass of 26,299 daltons. The deduced amino acid sequence revealed a proline-rich (42%) amino-terminal region containing a number of repeated sequences similar or identical to the sequence PGGSYPPPPP. The reactivity of four monoclonal antibodies (F47-9, F67-1, F67-5, and F126-5) was directed to this proline-rich region of the PRA protein. DNA sequence and immunological data indicated that the lambda gt11 recombinant Y3180, which was previously isolated by using antibody F47-9 (R. A. Young, V. Mehra, D. Sweetser, T. Buchanan, J. Clark-Curtiss, R. W. Davis, and B. R. Bloom, Nature (London) 316:450-452, 1985), specifies a fusion protein unrelated to PRA but containing a similar epitope recognized by F47-9.</p>},
  year = {1990},
  journal = {Infection and immunity},
  volume = {58},
  pages = {80-7},
  month = {1990 Jan},
  issn = {0019-9567},
  language = {eng},
}