@article{7162,
  keywords = {Biological Assay, Biopsy, Drug Resistance, Bacterial, Genes, Bacterial, Humans, Leprostatic Agents, leprosy, Mycobacterium leprae, polymerase chain reaction, Rifampin},
  author = {Sapkota BR and Ranjit C and Macdonald M},
  title = {Reverse line probe assay for the rapid detection of rifampicin resistance in Mycobacterium leprae.},
  abstract = {<p>Mutations in the rpoB gene of 40 biopsy isolates of Mycobacterium leprae were analyzed by reverse hybridization-based line probe assay after PCR, and nine distinct single-nucleotide substitutions were found. Among them, a 3-nucleotide substitution was found in two, and 2-nucleotide substitutions were found in seven isolates. This is a new finding of multiple mutations in a single point of the rpoB gene for rifampicin resistance. This investigation demonstrates that the pattern of mutations in the rpoB gene for rifampicin resistance in Nepal involves more variety.</p>},
  year = {2006},
  journal = {Nepal Medical College journal : NMCJ},
  volume = {8},
  pages = {122-7},
  month = {2006 Jun},
  language = {eng},
}