@article{7043,
  keywords = {Adolescent, Antigens, Bacterial, Carbon Dioxide, Glycolipids, Humans, leprosy, Lipids, Male, Mycobacterium leprae, Palmitic Acid, Palmitic Acids},
  author = {Shannon E J and Harris E B and Haile-Mariam H S and Guebre-Xavier M and Frommel D},
  title = {Competency of human-derived Mycobacterium leprae to use palmitic acid in the synthesis of phenolic glycolipid-I and phthiocerol dimycocerosate and to release CO2 in axenic culture.},
  abstract = {<p>Insufficient numbers of viable Mycobacterium leprae have hampered metabolic studies using human-derived M. leprae. In this study, sufficient numbers of M. leprae were obtained from an untreated lepromatous patient to titrate the effects of pH on the metabolism of 14C-palmitic acid by M. leprae. Catabolic metabolism (oxidation of 14C-palmitic acid and release of 14CO2) was maximal when M. leprae were incubated at 33 degrees C and suspended in Middlebrook 7H9, ADC supplemented medium that had been buffered to maintain a pH of 4.8. Anabolic metabolism (synthesis of 14C-phenolic glycolipid-I and its precursor, 14C-phthiocerol dimycocerosate) was maximal when the pH was maintained at 6.8.</p>},
  year = {1992},
  journal = {Leprosy review},
  volume = {63},
  pages = {101-7},
  month = {1992 Jun},
  issn = {0305-7518},
  url = {http://leprev.ilsl.br/pdfs/1992/v63n2/pdf/v63n2a02.pdf},
  doi = {10.5935/0305-7518.19920013},
  language = {eng},
}