@article{6663,
  keywords = {Animals, Antigens, Bacterial, Disease Models, Animal, Hypersensitivity, Delayed, Immunity, Cellular, leprosy, Lymphocyte Activation, Mice, Mice, Inbred Strains, Mycobacterium, Mycobacterium Infections, Spleen, T-Lymphocytes, Regulatory},
  author = {Watson S R and Collins F M},
  title = {Development of suppressor T-cells in Mycobacterium habana-infected mice.},
  abstract = {<p>Mice were infected intravenously with increasing numbers of Mycobacterium habana (simiae serotype II), and the levels of delayed-type hypersensitivity to purified protein derivative and M. habana cytoplasmic protein antigen were determined after 14, 30, and 90 days. A footpad delayed-type hypersensitivity response was seen in 14-day-infected mice and was followed by a persisting anergy. T-cell-enriched suspensions collected 30 and 90 days into the infection (anergic donors) showed depressed transformation indexes after phytohemagglutinin and M. habana cytoplasmic protein antigen treatment in vitro. The corresponding B-cell mitogen (lipopolysaccharide) responses were not affected. Mixing experiments with T-cell-enriched suspensions from day-90 M. habana-infected donors adoptively suppressed lymphocyte transformation by normal and day-14 spleen cells. This effect could be ablated by anti-theta serum and complement treatment of the day-90 cells, indicating that the lack of in vitro responsiveness to cytoplasmic protein antigen was mediated by a population of suppressor T-cells present in the heavily infected spleens. There was no evidence that similar cells were present in the spleens of the 14-day-infected animals. Suppressor T-cells could be induced in vitro by exposure of day-14 spleen cells to concanavalin A or M. habana cytoplasmic protein antigen before they were mixed with normal or day-14 indicator splenic lymphocytes. The timing of the appearance of suppressor T-cells in the infected spleens corresponded to a loss of footpad hypersensitivity by the M. habana-infected animals.</p>},
  year = {1979},
  journal = {Infection and immunity},
  volume = {25},
  pages = {497-506},
  month = {1979 Aug},
  issn = {0019-9567},
  url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC414480/pdf/iai00188-0021.pdf},
  language = {eng},
}